BIOL 123 Lab Manual

BIOL 123 Library

This reference page houses supplementary information on many of the topics we will cover.
You can also add your own valuable resources to help build our collection!
Add to our collection
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  • Intro to Science
    • Lab 1 Doing Science
    • Lab 2 Analysis
    • Lab 3 Peer Review >
      • Lab 3 Fall
      • Lab 3 Spring
  • Evolution
    • Lab 4 Evidence
    • Lab 5 Phylogenies
    • Lab 6 Taxonomy
  • Biodiversity
    • Lab 7 Showcase
    • Lab 8 Bioindicators
    • Lab 9 Metrics
  • Ecology
    • Lab 10 Principles
    • Lab 11 Policy
    • Lab 12 Blitz (Sp Only)
  • Library
​TERRESTRIAL ISOPODS: PILLBUGS & ISOPODS
Terrestrial isopods are arthropods. Although we call them "pillbugs" and "sowbugs," they are actually crustaceans that occur in moist, dark areas, such as: forest floors, leaf and grass piles, and under dead logs where they eat detritus.
fact sheet
Some good citations:
A preliminary study on habitat features and associated terrestrial isopod species
Learning from the environment: how predation changes the behavior of terrestrial Isopoda (Crustacea Oniscidea)
Evolutionary adaptation of oniscidean isopods to terrestrial life: Structure, physiology and behavior ​
Scientific Literature & Citations

Searching the Lit

Investigating the scientific literature on your topic is an important step of the scientific method; it connects your question to existing theory and research. Some of this was done for you so you could begin with your experiment last week. We need more research before we can proceed, and this will also give you a good start on your poster.
Scientific articles are often not free to the public, so large institutions, like universities, pay fees for access. If you are not on a campus computer, you will need to sign into the library using your WKU account to access some articles: Just click HERE, and search "Google Scholar" [see example].
If you are unsure if your article is actually peer-reviewed:
  • Use these 5 Ways to Tell If an article Peer-reviewed … or Academic, from the Prairie State College Library
  • Or this informative video from Vanderbilt University
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Link to Google Scholar.
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Library.
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Link to article.

Citations

Citation and style guidelines very by journal but are usually some type of modified-APA format. We will be following formatting guidelines of the Kentucky Academy of Science.
IN-TEXT CITATION OF LITERATURE
  • Cite publications in the text by author(s) and date – e.g., (Readley 1994)
  • Multiple citations should be  in chronological order and separated by semi-colons – e.g., (Foster, 1976; Ashley et al., 1987; Brown, 1994)
  • Multiple citations of works by one  author(s) should be in chronological order – e.g., (Jones 1978, 1983).
  • Publications by one author(s) in the same year should be distinguished by a, b, c, etc. – e.g., (Smith 1994a, 1994b).
  • For in-text references to works with one or two authors use names of both authors – e.g., (Jones and Williams, 1991).
  • For works with three or more authors use name of the first author followed by et al. – e.g., (Lee et al., 1985).
  • Do not include any reference unless it has been published or accepted for publication (“in press”; see below).
LITERATURE CITED [END OF DOCUMENT/PAPER/ASSIGNMENT] 
This section is also referred to as the "References" or "Works Cited" section. List all authors of each entry. Do not abbreviate journal titles. The first line of each reference should be typed flush left; the remaining lines should be indented five spaces. Examples of common types of references are given below.
  • JOURNAL ARTICLE (one author): Lacki, M. J. 1994. Metal concentrations in guano from a gray bat summer roost. Transactions Kentucky Academy Science, 55:124-126.
  • JOURNAL ARTICLE (multiple authors): Jones, D. G., Little, D. P., and Marks, L. J. 2010. White-tailed deer (Odocoileus virginianus) and Wasting Disease in Western Kentucky. Transactions Kentucky Academy Science, 55:124-126.
  • BOOK: Ware, M., and R. W. Tare. 1991. Plains Life and Love. Pioneer Press, Crete, WY.
  • PART OF A BOOK: Kohn, J .R. 1993. Pinaceae, Pages 32-50 in J. F. Nadel (ed). Flora of the Black Mountains. University of Northwestern South Dakota Press, Utopia.
  • WORK IN PRESS: Groves, S. J., I. V. Woodland, and G. H. Tobosa. In press, Deserts of Trans-Pecos Texas.
  • WEB PAGE: Smith, A.W. 1999. Title of web site. Web site address. Date accessed (06/12/2005).
Scientific method & theory
It's just a theory? Biological theories underpin all the concepts and principles explored in both lecture and lab. Testing a single hypothesis, even with repetition, cannot lead to a theory; it can, however, add to theoretical development.
Ask a question
Questions can arise from observations in the field, in the lab, from the scientific literature (e.g., scientific posters, presentations or papers), or from pre-existing data. In this class, sometimes your question will be provided to you and other times you will get to decide what questions you want to investigate.
Conduct background research
This is a literature review process, in which researchers dig deep into what is already known about their topic of choice and what questions still remain. Often, the literature review helps to refine questions and direct hypothesis formation. Background research will be provided to you in our first few labs, but you will also do some research on your own in later lab activities.
Construct a hypothesis
A quality hypothesis must be testable, objectively measurable, specific, and falsifiable. It must include a prediction and potential mechanism(s) based in the literature or from previous work. You will do this often in BIOL 123.
Test with an experiment
There are various approaches to research design, based on your research question. These range from purely descriptive to experimental designs, which involve manipulation of a variable or variables. Regardless of the method selected, the design should have clearly identified variables by type, and should be both valid and reliable. You will have varying levels of input on the experiments we conduct in lab.​
Analyze the data & draw conclusions
The methods used for analysis are largely based on the research design. In the biological sciences, analysis almost always involves the use of statistical tests and graphical representations of data. We will use several different types of statistics throughout this course.
Communicate your results
This last step is essential. For our understanding of the natural world to grow, new research must be shared so others can draw on what is known to expand our understanding. Results can be communicated through technical reports, presentations at conferences, scientific posters, and manuscripts which appear in scientific journals. You will create many of these products throughout BIOL 123
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Analysis: Excel & Statistics

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Click here to review the "White Squirrel Scenario" for help with chi-square.
Chi-Sq Contingency Calculator

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Click for link to t-test calculator.
T-test video instructions
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Review our songbird scenario for an example using a t-test for statistical analysis.

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Simpson's Diversity Index formula, where n = number of individuals of each species N = total number of individuals of all species
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SDI Calculator
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Both these communities have the same abundance (16 trees). They both have the same richness (4 types of trees = 4 species). But they have different evenness. Community 1 has an "even distribution" of species (all four are in the same amount), whereas community 2 is dominated by one species. Click to enlarge.
BioDiversity & Watershed Health

Resources to get you started

Inciators of biodiversity
biodiversity & ecosystem function
Assessing watershed health
Protists as bioindicators
Agricultural Stream health

Governmental & NGO Resources on the green river, KY.

Energy & Environmental Cabinet of KY
KY Waterways Alliance
The Nature Conservancy
Microscopy
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Diagram of a compound microscope. The magnification and compound nature (two lenses) make these more powerful. We will use binocular compound scopes in lab, meaning they have dual optical lenses, one for each eye.
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Diagram of a dissecting microscope. These have an external light source, but can be used in the field with light from the sun, making them a valuable tool in vivo.
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Quiz Link. Use this link to practice microscope part ID. The scope used is monocular and ours will be binocular, but the rest of the parts will be the same.
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Click to enlarge.
Written and collated by Natalie Mountjoy & Steve Huskey
This website is intended solely for use of BIOL 123 students at Western Kentucky University. Usage for any other persons is expressly prohibited. The information here is copyrighted (all rights reserved ©), cited, or within "Fair Use" under the scholarship or education exemption (section 107 of the Copyright Act).
BIOL 123 Online Lab Manual © 2022 by Natalie Mountjoy is licensed under CC BY-NC-SA 4.0 
  • Home
    • About
    • Safety
    • Suggestions
  • Intro to Science
    • Lab 1 Doing Science
    • Lab 2 Analysis
    • Lab 3 Peer Review >
      • Lab 3 Fall
      • Lab 3 Spring
  • Evolution
    • Lab 4 Evidence
    • Lab 5 Phylogenies
    • Lab 6 Taxonomy
  • Biodiversity
    • Lab 7 Showcase
    • Lab 8 Bioindicators
    • Lab 9 Metrics
  • Ecology
    • Lab 10 Principles
    • Lab 11 Policy
    • Lab 12 Blitz (Sp Only)
  • Library